动物造模,脂肪间充质干细胞 z-bio 2021-06-23 528

　　Purpose: Separation? Do you want to induce multidirectional differentiation and identify ferret adipose-derived mesenchymal stem cells?

　　Method: Aseptically collect ferret abdominal subcutaneous fat, mechanically cut to 0.5mm, digest with 0.075? Proenzyme I, smear high sugar? DMEM medium (add 4g)/mLβ? Have you performed FGF) culture, observation of cell morphology, flow cytometry analysis of cell surface antigen marker expression, and in vitro adipogenesis, bone formation, cartilage differentiation potential detection, and further attempts at neural differentiation?

　　Result: The adhesion and proliferation of fibroblast-like cells can be separated from fresh adipose tissue by using tissue mass attachment method. The results of flow cytometry showed that CD29 (53.1?, CD90 (99.6? and CD105 (93.7? High expression., CD11b (36.3?, CD45 (28.3? Low expression? Can these cells be induced to differentiate into fat, bone, Cartilage and neurons?

　　Conclusion: Adipose-derived mesenchymal stem cells are isolated from ferret adipose tissue. Do these cells have multi-directional differentiation potential?