动物造模 z-bio 2021-11-03 282

　　Purpose: Have you observed normal characteristics of weight gain and loss that affect blood sugar levels?

　　Methods: 8-week-old male C57BL/6 mice were fed a high-calorie diet (60? fat calories) for 8 weeks to present an obesity model: weight intervention group (intervention group, IT) and obesity group were randomly assigned to (obesity group, OB), obesity Does the group stick to a high-calorie diet to the end? Can the control group (CON) eat normally? Restricting calorie intake caused the IT group to lose weight to the level of the CON group (P<0.05), and then resume the high-calorie diet to rebound the weight to the level of the OB group. The tolerance test reflects the level of blood glucose metabolism, and the insulin resistance test evaluates insulin sensitivity. Pancreatic pathology HE staining to observe the morphological changes of pancreatic islets, do you do?

　　Result: The weight gain rate in the IT group was 2.27 times higher than that before the intervention, and the fasting blood glucose level was 5.13 times higher. Compared with the OB group, the area under the blood glucose curve of the experimental end-point glucose tolerance test increased, and insulin sensitivity was significantly reduced (Pu003c0.05). Pu003c0.05), increased visceral fat (Pu003c0.05), and the area of islets is significant. (Pu003c0.05)?

　　Conclusion: Weight fluctuations lead to increased visceral fat? Impairment of insulin sensitivity and impaired glucose tolerance?