动物造模,大鼠阴道涂片 z-bio 2021-06-24 616

　　Objective: To observe the cell morphology by staining the vaginal smear of SD female rats, and to find a simple staining method to determine the four stages of the rat estrus cycle?

　　Method: 20 animals weigh 216? Yes it is. Use a wet cotton swab to insert 0.5-1.0 cm into the vagina of the female mouse. Gently overlap 1-2 times, drop on a clean glass slide, add saline, and air dry, then quickly perform Gram staining and Crystal Violet staining to distinguish the estrus cycle of female rats under an optical microscope. can you?

　　Result: Is the cell nucleus in dark purple, cytoplasm in light purple, and white blood cells in dark purple? Rapid Gram staining stains the nucleus purple, the cytoplasm becomes rose, and the white blood cells become dark red. The average estrus cycle of SD female rats is 4-5 days, before estrus? estrus? 4 stages of post-estrus and estrus? In the pre-estrus stage, mainly nucleated epithelial cells, during the estrus period, almost all anucleated keratinocytes can form clusters; in the late estrus period, there are many white blood cells but few nuclear keratinocytes; during the estrus period, white blood cells, there are nucleus and anucleated keratinocyte Do the forming cells, mainly white blood cells, exist at the same time? Both staining methods are relatively easy to operate and can distinguish the four stages of the estrus cycle of SD rats. The cell morphology of crystal violet staining may be slightly deformed, and the cell morphology of rapid gram staining is complete and the image is clear. Is it fixed in absolute ethanol for a short time?

　　Conclusion: Rapid Gram staining does not change the reproduction of SD female mice. Is it easy to circulate? Can you quickly distribute the different stages of the emotional cycle and facilitate it when conducting large-scale experiments?