动物造模 z-bio 2021-10-07 746

　　Objective: To explore the best breeding and identification method for vitamin D receptor gene knockout mice, and to provide animal models for further research on VDR function and related diseases?

　　Methods: Early construction of the VDR knockout mouse research group. At the mouse stage, four different mating methods were used to produce offspring mice. The phenotype and genotype of the offspring were counted, and the VDR knockout homozygotes were obtained (compare VDR). -/- breeding)) mice and heterozygous (VDR +/-) mice. Do you want to analyze the expression of VDR at both mRNA and protein levels at the same time?

　　Result: VDR-/-Mice and VDR +/- mice have important phenotypes, which are mainly manifested as hair loss, small size, and decreased mobility. The offspring mice produced by the four breeding methods are basically in line with Mendelian inheritance; VDR-/- mating mice have low fertility, which directly expands the VDR-/- knockout mouse lineage and cannot be used. This method is the best way to expand the pedigree, because the VDR+/- interaction method has been successfully obtained in VDR-/- mice, and it has been detected that the VDR gene knockout trait is stably inherited in offspring mice. is it?

　　Conclusion: Have you successfully identified the genotype of the offspring of the experiment and established a VDR gene knockout mouse strain, laying the foundation for subsequent VDR function research?