动物造模 z-bio 2021-06-24 421

　　Purpose: Do you want to screen the microsatellite loci suitable for testing the genetic quality of cats?

　　Method: Select 74 microsatellite sites from relevant literature and GenBank, investigate the optimal annealing temperature to optimize the PCR reaction conditions, and perform agarose gel STR scanning results based on the results of gel electrophoresis and agarose gel. stable? Are your alleles rich? Is it a set of microsatellite loci evenly distributed in 18 pairs of cat autosomes for testing the genetic quality of experimental cats?

　　Result: From the 74 candidate microsatellite loci, are 55 polymorphic and stably amplified STR loci selected? Then, 1-2 polymorphisms were correctly selected for each cat's autosomes (excluding A1 and B1 chromosomes).

　　Conclusion: Is the selection of microsatellite loci suitable for analyzing the genetic structure of cat populations successful?