动物造模 z-bio 2021-06-24 534

　　Objective: How to explore the feasibility of PLCζ as an activator and its effect on oocyte fertilization and embryo development after ROSI?

　　Method: Construct the recombinant plasmid pCRII-TOPO-PLCζ in vitro, and then use mass spectrometry identification and Western blot to detect the antigenicity after expression induction? Recombinant PLCζ Is this protein used as an activator of mouse ROSI oocytes to record fertilization and embryogenesis and statistical analysis?

　　Result: The recombinant plasmid pCRII-TOPO-PLCζ was successfully constructed and was identified as a mouse after expression in vitro. PLCζ protein determined by mass spectrometry. Do you have good antigenicity? Will recombinant PLC ζ protein activate the fertilization rate of oocytes? 2-4 frame rate? 8 The cell rate and cyst formation rate are not significantly different from the blank group (P→0.05)?

　　Conclusion: The recombinant PLC ζ protein in this study has no significant effect on oocyte fertilization and embryo development. Is its feasibility as an oocyte activator after OSI in mice questionable?