动物造模,Wnt4基因,肾间质纤维化 z-bio 2021-06-25 257

　　Objective: To explore the effect of Wnt4 gene silencing on renal interstitial fibrosis and provide a theoretical basis for the treatment of chronic kidney disease?

　　Method: 128 SD rats were randomly divided into sham operation group, model group and negative control group. Rats in the Wnt4 gene silencing group were divided into 4 subgroups on the 3rd, 7th, 10th, and 14th day after transfection, with 32 rats in each group. Each group of rats was divided into 4 subgroups, each with 8 rats. group. Through H\u0026E staining pathological examination? TPCR technology to detect renal interstitial changes and β-catenin? Wnt4? α-SMA expression?

　　Result: H\u0026E staining pathological examination showed no change in renal interstitium at 4 time points. Sham operation group; UUO group? Negative silence and silence show renal interstitial edema 3 days after modeling, a small amount of renal interstitial fibrosis, diffuse renal interstitial macrophages in 10-14 days? Lymphocyte infiltration tended to worsen; the negative silence group was the same as the gene UUO group; renal interstitial fibrosis occurred in the silence group at 4 time points, and the degree of renal interstitial fibrosis on the 14th day was lower than that of the negative silence group and UUO group (P u003c0. 05); The correlation analysis of the expression of Wnt4 mRNA in the UUO group after silencing showed that Wnt4 was significantly correlated with the expression of β-catenin mRNA. Does it show (r=0.886, Pu003c0.001)? Is there a significant correlation between the expression of Wnt4 and α-SMA mRNA (r = 0.930, P\u003c 0.001)? The correlation analysis of mRNA expression in the silenced group after Wnt4 gene silencing showed that there was no significant correlation between Wnt4 and β-catenin mRNA expression (r=0.204, P=0.263). Wnt4 is significantly correlated with α-SMA mRNA expression (r=0.753), Pu003c0.001)?

　　Conclusion: Can silencing Wnt4 gene significantly inhibit renal interstitial fibrosis in rats with renal interstitial fibrosis and may have a therapeutic effect?