动物造模 z-bio 2021-06-25 293

　　Purpose: Do you want to annotate the histone modification sites in the entire Tibetan minipig genome and compare the differences in histone modifications between different tissues?

　　Method: Collect two 3-month-old male non-fetal Tibetan mini pigs. Collect blood and liver. , Small intestine (ileum) and longissimus dorsi tissue samples, perform chromatin immunoprecipitation experiments, capture DNA fragments for high-throughput sequencing? The off-machine data is filtered and compared with the reference genome. Use MACS software to calculate read richness. On the genome?

　　Result: Different histones have different priority positions for the distribution of gene promoter regions, and the distribution rules can be roughly divided into two categories. In one type, such as H3K4me3, histone modifications are mainly distributed bilaterally. At the transcription start site, there is a narrow, clear peak near the downstream TSS. Other types of histone modifications, such as H3K9me3, are more evenly distributed throughout the promoter region, showing an overall appearance. The low peaks on the inner side and the high side; the genes affected by tissue-specific histone modifications are different, and the corresponding functional pathways are also relatively extensive, but are they all involved in the transcriptional regulation of the RNA polymerase II promoter?

　　Conclusion: Does this study provide basic data for future transcriptional regulation studies using Tibetan minipigs as a biomedical model for the histone modification annotation data of the four tissues of Tibetan minipigs?