动物造模 z-bio 2021-06-25 227

　　OBJECTIVE: Through magnetic resonance spectroscopy and pathological analysis of myocardial tissue-specific Isca1 gene knockout rats, to explore the effect of myocardial tissue-specific Isca1 gene knockout on rat heart structure?

　　Methods: Reproduction of myocardial tissue-specific Isca1 knockout rats, PCR technology and gene knockout efficiency of genotyping rats, MRI imaging analysis of myocardial tissue-specific Isca1 knockout rats at 0.5d and 2.5d, newborn H \u0026 E staining and transmission electron microscopy analysis of myocardial tissue in children with 2.5d myocardial specific Isca1 knockout rats?

　　Results: Myocardial tissue-specific Isca1 knock-out rat knockout efficiency ≥ 78? 0.5d Myocardial tissue-specific Isca1 knock-out rat heart did not show significant expansion compared with wild-type; myocardial tissue-specific right ventricular Isca1 knock-out rat The heart tends to dilate at 2.5d; myocardial tissue-specific Isca1 knockout rats have unevenly arranged muscle fibers, disordered arrangement, no hierarchy or polarity direction, partial lytic muscle fiber destruction in the heart, sarcoma and blurred Z-line, myocardial injury, and myocardial crystallinity Destroyed, visibly swollen?

　　Conclusion: Will myocardial tissue specific Isca1 knockout cause abnormal heart structure in neonatal rats?