动物造模,Fancm 基因敲除小鼠 z-bio 2021-10-12 257

　　Objective: To construct Fancm gene knockout mice of Fancmi anemia pathway and study the effect of Fancm gene deletion on the physiological functions of mice, especially the physiological functions of male reproductive organs?

　　Methods: CRISPR/Cas9 technology was used to analyze the wild-type and Fancm-/-FANCM protein expression in the testis of Fancm-/-Fancm-/- mice, and calculate the fertility rate. Offspring and analysis of blood routine indicators. Histomorphological research Male physiological and pathological phenotype Fancm-/-Mouse Testis?

　　Result: Knock out the ATG region of the Fancm gene, and get a stable hereditary C57BL/6 background Fancm-/-mouse? Does Fancm-/-mouse testis fully express FANCM protein? Fancm-/- mice have no obvious embryonic lethality, but is the number of female Fancm-/- mice significantly lower than that of male Fancm-/- mice? The body weight of Fancm-/- mice in the same litter was not significantly different from that of the wild type. , Are there significant differences in some blood routine indicators? Do Fancm-/- mice have obvious reproductive disorders? Do male Fancm-/- mice have obvious testicular development disorders and increased spermatogenic cell apoptosis? Does it affect cell cycle arrest, testicular development and sperm production?

　　Conclusion: Stable gene C57BL/6 Background Fancm-/- successfully obtained mice. Does Fancm gene participate in the growth and development of mice, especially the maintenance and regulation of male reproductive organs?