动物造模 z-bio 2021-06-25 703

　　Objective: To study the effect of sodium fluoride on histopathology of bone, brain, liver and kidney in rats

　　Method: 20 healthy weaned SD rats, half male and half male, were randomly divided into control group and fluorosis group. Rats in the fluorosis group freely drank tap water containing sodium fluoride (fluoride ion concentration 100mg/L), weighed regularly, measured body length and tail length, painted teeth with fluoride, and observed the symptoms of fluorosis and addiction. Test every 12 weeks after each feeding. Measure the fluorine content in femur, liver, kidney, and brain tissues of rats in the group, and measure the malondialdehyde (MDA) content in serum, liver, kidney, and brain tissues. Observe the structural changes of each histopathology under light microscope and electron microscope?

　　Result: The food intake, water consumption, body weight, body weight and tail length of the fluoride-containing food control group were slightly lower than those of the control group, but the difference was not statistically significant. The rats in the control group did not have dental fluorosis, the incidence of dental fluorosis in the dental fluorosis group was 100? Compared with the control group, the content of femur, liver, kidney and dental fluorosis in the rats in the dental fluorosis group was significant. Increase (Pu003c0.01). Are the levels of MDA in serum, liver, kidney, and brain tissues of rats in the fluorosis group higher than those in the control group (Pu003c0.01 or Pu003c0.05)? Does the light microscope result show that the thickness of the trabecular bone of the rats in the fluorosis group is uneven? The interval is widened; some liver cells are obviously edema, and the liver cells are dislocated; the composite renal tubular epithelial cells near the renal cortex swell, and the lumen is enlarged. Are the hippocampal neurons swelled into vacuoles? In the fluorosis group, the electron microstructure, osteoblasts, hepatocytes, proximal tubular epithelial cells, and brain neuron superstructures all showed nuclear pyknosis, chromatin condensation, mitochondrial swelling, and vacuolation. Are they typical Features of apoptosis? Conclusion Can sodium fluoride cause pathological damage to the bone, liver, kidney and brain tissues of experimental rats and cause cell apoptosis?