动物造模,Zucker糖尿病肥胖,脑血管病变 z-bio 2021-10-08 920

　　Objective: To observe the pathological characteristics of cerebrovascular disease in Zucker diabetic obesity (ZDF) rats, and to preliminarily explore its etiology, so as to provide experimental and theoretical basis for the application of ZDF rats?

　　Method: 8 male ZDF rats of 8-9 weeks of age were fed Purina #5008 diet for 12 weeks, and the other 8 male Zucker lean (ZL) rats of the same age were fed a normal diet as a normal control group. The group tested body weight, fasting blood glucose and glycosylated hemoglobin (HbA1c), used Y maze to evaluate the cognitive function of rats, observed the overall shape of intracranial blood vessels through magnetic resonance imaging, and collected animal brains after the victim The organization was sacrificed. Observation through conventional pathology and ultrastructural pathology. Do you use the large and microvascular lesions in brain tissue and immunofluorescence technology to observe the expression of albumin, IgG, tight junction protein occludin and ZO-1?

　　Results: Compared with normal controls, ZL rats have significantly increased body weight, fasting blood glucose, and HbA1c in 20-week-old rats (Pu003c0.01). The new group Y labyrinth experiment time was significantly reduced (Pu003c0.05); segmental stenosis with large blood vessel malformations and nodules in the brain; cerebral microvascular obstruction, peripheral nerve fiber edema; perivascular albumin and IgG exudation, ZO- 1 and occludin protein expression decreased (Pu003c0.01))?

　　Conclusion: ZDF rats are 20 weeks old. Cognitive dysfunction, intracranial large and microvascular stenosis, obstruction, and peripheral nerve tissue diseases occur. The cause is the lack of tight junction proteins, which leads to the destruction of blood-brain barrier function, increased permeability and vascular morphology. And functional lesions?