动物造模 z-bio 2023-02-01 784

　　Objective: How to establish a mouse anti-rabies virus IgG antibody ELISA detection method for the detection and analysis of rabies mouse models?

　　Method: Use orthogonal experiments to determine the best working conditions such as the best dilution of the sample and the secondary antibody; and analyze the specificity, sensitivity, stability, etc. of the method; use it in conjunction with a commercially available kit for detecting mouse samples, Determine the coincidence rate of ELISA?

　　Result: The sequence of the ELISA method is 1:100, the optimal concentration of the secondary antibody is 40g/mL, and the positive cut-off value is 0.121? This ELISA method does not cross-react with virus-positive sera commonly found in mice. The lower limit of the detection concentration is 129 μg/mL, and the coefficient of variation of the sample for 3 replicates is less than 10? Is the matching rate with the commercial kit 100?

　　Conclusion: ELISA detection of mouse anti-rabies virus IgG antibody has successfully established a suitable method for rabies mouse model analysis and vaccine titer evaluation?