动物造模 z-bio 2021-10-12 459

　　Purpose: To collect the measurement results of peripheral blood lymphocyte subsets completed by the National Center for Drug Safety Evaluation and Monitoring during 2007-2019 in accordance with the Good Laboratory Practice (GLP) conditions of non-clinical drug quality control standards. Do (GoodLaboratoryPractice), GLP), and establish BALB/c mice? What is the normal reference range for SD rats and cynomolgus monkeys?

　　Methods: Using heparin sodium anticoagulation tube to collect animal peripheral blood, 303 BALB/c mice, 359 SD rats and 460 cynomolgus monkey blood samples containing different fluorescent antibodies were compound stained and then flow cytometry was used to detect CD3+ expression. Lymphocytes are counted, CD4 +, CD8 + and CD20 + (only for cynomolgus monkeys). Do you want to calculate the normal reference range and 95 confidence interval for age (rats and mice) separately?

　　Results: The ratio of CD4+/CD8+ lymphocytes in BALB/c mice ranged from 1.07 to 8.59; the ratio of CD4+/CD8+ lymphocytes in SD rats ranged from 0.54 to 11.67; the ratio of CD4+/CD8+ lymphocytes to 3-4 years old crab hair and CD3-CD20 + The percentage of lymphocytes ranges from 0.35 to 4.22 and 1.40 51.99?

　　Conclusion: Lymphocyte subgroup classification number is an important reference index for product immunotoxicity evaluation. Does the collection and establishment of relevant background data help to make a reasonable toxicity assessment of the drug?