动物实验 z-bio 2021-06-29 1095

　　OBJECTIVE: To investigate and compare the various injury patterns and rules of CBA/CaJ and C57BL/6J mouse cochlear hair cells with age.

　　Method: Select 32 CBA/CaJ and C57BL/6J mice at 6 months, 12 months, 18 months, 24 months, 8 years old, and remove the cochlea under general anesthesia. The results of the number of hair cells inside and outside the cochlea were input into the computer, and the cochlear chart software was used to compare the cochlear hair cell density of the two kinds of mice according to the corresponding different ages, and I conducted an analysis.

　　Result: CBA/CaJ mice began to show mild loss of upper cochlear outer hair cells when they were 12 months old. With age, the loss of outer hair cells continues to increase, mainly from top to middle, and then to inner hair cells. Lose. Starting from 18 months of age, the loss of cochlear outer hair cells increased significantly compared with the previous age group (Pu003c0.05). In C57BL/6J mice, the outer hair cells and inner hair cells at the bottom of the cochlea were lost within 6 months, mainly outer hair cells, but then as the age grew, the loss of hair cells increased, and I continued to do this. The loss of inner hair cells was significantly higher than that of CBA/CaJ mice of the same age (Pu003c0.05), and the loss of inner hair cells was significantly higher than that of the pre-age group of mice of the same age (Pu003c0.05) .05). 12-month-old seeds.

　　Conclusion: CBA/CaJ mouse cochlear hair cell damage follows a pathological law, starting from the top and bottom to the center, but the upper hair cell damage is more serious than the bottom. The injury of C57BL/6J mouse cochlear hair cells follows the pathological law, starting from the lower part of the cochlea and gradually extending to the middle and upper part of the cochlea. Outer hair cells are damaged faster than inner hair cells. C57BL/6J mice have faster hair cell damage than CBA/CaJ mice. Although both types of mice are recommended as animal models for senile hair cell apoptosis research, the C57BL/6J mouse model is suitable for short-term observation and research. The difference in hair cell changes between the two may be related to whether the Cdh23 gene is mutated or missing.