动物模型,脂肪肝 z-bo 2020-08-07 573

　　(1) Replication method Adult rats are anesthetized with ether and inserted into the internal jugular vein with a 0.9mm internal diameter silicone tube. The catheter is subcutaneously pierced from the back of the neck and fixed, and the catheter is connected to a computer infusion pump. Put the animal in the metabolic cage and allow it to move freely. Daily non-protein calorie 213.4kJ (501kcal), nitrogen content 366mg, fat emulsion provides 35% of calories, 24h constant rate perfusion, until the composition of intravenous nutrient solution is The daily intake of 1276kJ (350kcal) per kilogram of body weight and glucose accounted for more than 83% of the total intake of calories. One week later, the animals were sacrificed, the liver was taken out and its wet weight was weighed, part of the tissue was weighed to prepare a homogenate to detect its TG and TC content, and the remaining tissues were fixed and stained for histomorphological examination.

　　(2) Model characteristics: The wet weight of the liver increases, the contents of TG and TC in the liver are increased, and the liver cells have obvious fatty infiltration.

　　(3) Comparative Medicine Steatosis is a common clinical complication of TPN. This model is similar to humans, but due to its complicated operation, its promotion and application are restricted.