动物造模,肝细胞损伤 z-bio 2021-10-09 633

　　Objective: To identify different proteins in rat serum after ketoconazole (KTZ) and carbon tetrachloride (CCl4) induced hepatocellular injury.

　　Method: A total of 96 7-week-old Wistar male rats were selected and randomly divided into 32 rats in the KTZ group, 32 rats in the control group, and 32 rats in the CCl4 group. The KTZ group took 225 mg/kg KTZ orally twice a day for a total of two doses. The control group was given the same amount of normal saline once orally. The CCl4 group received a single mandatory oral dose of 10 mL/kg of CCl4 (V/V: 30°. 4, 24, 48, and 72 hours after the end of the administration, the animals were anesthetized to collect blood, prepare serum, and prepare pathological sections with the liver. Gel electrophoresis (two-dimensional gel electrophoresis, 2-DE) uses KTZ and CCl4 serum to have obvious liver cell damage. The control serum is used for protein extraction, and then combined with mass spectrometry to identify different proteins.

　　Results: 24 hours after the end of the administration, the alanine aminotransferase (ALT) of the animals in the KTZ and CCl4 groups increased, and the liver cells had pathological changes. The difference was statistically significant (P). u003c0.05). Compared with the control group, the KTZ and CCl4 groups identified four different serum proteins, including apolipoprotein AI, plasma glutathione peroxidase, apolipoprotein E and vitamin D binding protein.

　　Conclusion: The four different proteins identified can be used as potential biomarkers of liver cell damage for further study.