动物造模 z-bio 2021-09-26 435

　　Objective: To study the age-related changes in gene expression in the subventricular zone (SVZ) of young, adult, and old mice.

　　Method: Based on transcriptome sequencing and real-time fluorescent quantitative PCR technology, SVZ mRNA was extracted from C57 mice aged 3 weeks, 6 months, and 20 months, and differential treatment was performed according to the expression of SVZ gene. Different age groups. Detection.

　　Result: Taking young mice as control, 253 differentially expressed genes were detected in adult mice, 519 differentially expressed genes were detected in old age, and 147 differentially expressed genes were detected in adult mice as control. ...In GO analysis, compared with young mice, the differentially expressed genes of adult mice are mainly related to nutrition and metabolism, while compared with adult mice, the genes that are differentially expressed are mainly related to the central nervous system. We found that it is involved in the neural differentiation of mice. KEGG analysis showed that the differentially expressed genes are mainly involved in protein digestion and absorption and neuroactive ligand-receptor interactions.

　　Conclusion: This study provides data on genetic changes in SVZ transcription levels at different ages, discusses the functional development and degeneration of SVZ, and provides a new theoretical basis and method for further treatment and resistance to neuro-aging.