动物造模,自发性2型糖尿病 z-bio 2021-07-02 325

　　Objective: To dynamically observe the pathological changes and characteristics of the liver of spontaneous type 2 diabetic db/db mice, and use the db/db mouse liver as a type 2 diabetic liver injury model to provide a basis for experimental research on basic theories and experiments?

　　Method: 7-8 weeks old, male, SPF db/m mice as the control group, diabetic db/db mice as the model group, 16? Observe weight dynamically? Fasting blood glucose (FBG)? Have you calculated the liver index of serum alanine active alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and observed the pathological changes of the liver with light and electron microscopes?

　　Result: The blood sugar, serum ALT and AST activities of fasting db/db mice were significantly higher than those of db/m mice. Histological examination found that the liver of db/db mice was severely damaged at 16 weeks of age, mainly manifested as fatty degeneration of hepatocytes and inflammatory cell infiltration. The lesion deteriorated within 32 weeks, and a large number of collagen fibers in the liver tissue can be seen under the microscope. Structures such as mitochondria and endoplasmic reticulum are destroyed or eliminated under an electron microscope, revealing transparent lipids. Do droplets, inflammatory cells and tissues contain a lot of collagen fibers?

　　Conclusion: The liver of db/db mice has obvious seborrhea and inflammatory cell infiltration at 16 weeks of age, and obvious fibrosis at 32 weeks of age. , db/db mouse liver can be used as an experimental study. Is the liver disease model of type 2 diabetes excellent?