气滞血瘀 z-bio 2021-09-29 250

　　Objective: To use gene chip technology to study the complete gene expression profile changes in the tongue of rats with congestion syndrome, the biological processes and pathways involved in various genes, as well as the theories and medications related to congestion syndrome.

　　Method: Adopt a high-fat diet and chronic unpredictable stimulation to reproduce the animal model of stagnation and hyperemia, and use gene chip technology to change the relevant route with the overall gene expression profile of the normal tongue. Model rat.

　　Results: Compared with normal rats, stagnant and congested rats showed 277 differentially expressed genes, of which 68 were up-regulated genes and 209 were down-regulated genes... GO and pathway analysis showed that Qi stagnation and blood congestion syndrome included inflammation, 7 pathways for changes in biological processes such as lipid metabolism, immune response, complement and coagulation cascade pathways, and PPAR signaling pathways. Cytochrome P450 has been shown to be related to changes in the metabolic pathways of foreign bodies.

　　Conclusion: Various genes in CYP450, complement and coagulation cascade pathway, and PPAR signaling pathway may be involved in the pathogenesis of congestion syndrome, providing a basis for the study of congestion syndrome and related drugs.