动物造模,生理性衰老 z-bio 2021-07-03 619

　　Purpose: Physiological aging leads to the down-regulation of the immune function of the body, thereby reducing the immune response of the elderly to infection and increasing their susceptibility to new sudden infections (such as severe influenza virus). Will the clinical symptoms become more severe after infection, and will the prognosis worsen? Therefore, has the research team established an elderly mouse model that is clinically compatible with severe influenza virus infection to evaluate the immune response characteristics of elderly host infection?

　　Method: Have you infected H7N9 (highly pathogenic) with various powerful influenza viruses? C57 mice (18-24 months old) were infected with H9N2 (low pathogenicity) in the nasal cavity. The changes in body weight and survival rate after infection were analyzed, and the lung virus replication and the expression of inflammatory factors were analyzed. ., And then perform a dynamic analysis of the lungs.

　　Result: Compared with adult control mice (6-8 weeks old), older mice were infected with H9N2 after losing weight for 7 days (only adult control decreased). The survival rate is reduced to 50? Adult control is 100?? The replication of H9N2 virus in the lungs of elderly mice is relatively high (Pu003c0.01), reaching a peak value (3.2x104 lung RNA virus copies/g) 2 days after infection. IL6 chemokine MCP1 and inflammatory factors are overexpression of pneumonia. The disease means 100-1000 times more than the adult control group; the pathological staining of the lungs is more serious, and the lung damage of the elderly mice is more serious, and the repair time after infection is longer, will it?

　　Conclusion: Have you successfully established an older mouse infection model that can replicate clinically transmitted immune responses in terms of infection survival, lung virus replication, and inflammatory damage? Is it used to better understand the mechanism research and evaluation of anti-infective drugs related to host infection in the elderly?