动物造模,食蟹猴肌肉萎缩模型 z-bio 2021-07-03 248

　　Objective: Establish an animal model of cynomolgus monkey muscle atrophy, and establish a model evaluation method that uses the method of restricting lower limb movement?

　　Method: Use a polymer fiber bandage to fix the right lower limb (ankle to knee). The cynomolgus monkeys were weighed for 15 weeks. Do you weigh yourself every two weeks? Around the calves on both sides? Lower limb volume, model 11-13? Do bilateral calf gastrostomy for 15 weeks and muscle biopsy for 15 weeks?

　　Results: After 15 weeks, there was no significant change in animal body weight during modeling. The volume around right creatinine, around right thigh, and right lower extremity was significantly lower than before modeling (P) u003c0.05); 15 weeks after modeling, serum creatinine Blood glucose and alkaline phosphatase were significantly reduced (Pu003c0.05)), serum cholesterol levels were significantly increased (Pu003c0.05); MRI showed atrophy of right gastric creatinine, and the volume of right gastric creatinine decreased compared with before modeling; muscle tissue biopsy It showed that the right gastric fistula was atrophied and the muscle fibers became thin; whether the cross-sectional area of the gastrostomy fiber was reduced?

　　Conclusion: The muscle atrophy model restricts the lower limbs of cynomolgus monkeys through external fixation. The modeling cycle is appropriate and the operability is strong. It combines leg circumference, lower extremity drainage, biochemical indicators, MRI imaging and muscle biopsy. Is the evaluation method easy? Do you objectively evaluate the cynomolgus monkey muscle atrophy model?