动物造模,动物模型 z-bio 2021-10-14 547

　　OBJECTIVE: To investigate the pathogenic mechanism of animal air pollution, and to establish an animal model of air pollution caused by inflammation in BALB/c mice through non-invasive tracheal infusion of PM2.5 particle suspension.

　　Method: 150 SPF BALB/c mice were divided into blank control group, saline group, low PM2.5 group (2.5mg/kg), moderate PM2.5 group (5mg/kg), high PM2.5, They were randomly divided into several groups (10 mg/kg). There were 5 groups in total, and each dose group received tracheal infusion on days 3, 7, 21, 35, and 49. Tissue samples and lung tissues were taken 24 hours after the tracheal infusion operation was completed. Use ELISA for pathological HE staining. How to verify the feasibility of non-invasive tracheal infusion and whether the inflammation model has been successfully established?

　　Result: Is the success rate of this modeling method 96%? Tracheal infusion was used to simulate the inflammation of lung tissue in mice. The score is positively correlated with airway injection time and dose extension. After exposure to PM2.5, a large number of lymphocytes and macrophages infiltrate the phagocytic particles of the lung, thereby increasing the alveolar space. Compare each exposure group with a normal saline control group. Compared with the blank group, does the inflammatory factor IL-6 in the alveolar lavage fluid and the TNF-α level in the lung tissue homogenate increase, and the high-dose group has the biggest difference?

　　Conclusion: In this experiment, we established mice that caused inflammation through tracheal infusion. The model has been successful, which proves that the method is simple and reliable. Can be widely used for repeated infusions. Is the study of the mouse respiratory system useful for further research on air pollution and other inflammatory mechanisms?