动物造模,异种移植模型 z-bio 2021-10-22 761

　　Objective: To use the PDX model of pancreatic cancer to evaluate the efficacy of clinical chemotherapy and to screen personalized treatment plans.

　　Method: Transplant fresh surgical specimens of pancreatic cancer subcutaneously into nude mice to establish a PDX model and pass it stably. STR genotyping is used to detect the traceability of tumor tissue in the PDX model. For clinical use, choose oxaliplatin, gemcitabine or iritinib. Kang treated three chemotherapy drugs and measured the tumor volume. The plasma CA19-9 test was added to evaluate the therapeutic effects of these three chemotherapy drugs using a mathematical model of the TGD level.

　　Result: The traceability of tumor tissue samples in the PDX model is 99?9? which is consistent with the primary tumor. Compared with the control group, both the irinotecan and gemcitabine treatment groups showed significant therapeutic effects (P = 0.001), gemcitabine showed more obvious anti-tumor effects, irinotecan was the most toxic, followed by gemcitabine. I will.

　　Conclusion: We have successfully established a PDX model of pancreatic cancer and passed it stably. The mathematical model of TGD levels indicates that gemcitabine plays the most important role in inhibiting tumor growth.