动物造模,肠系膜上静脉血栓形成大鼠模型 z-bio 2021-11-25 833

　　Objective: To establish a rat model of superior mesenteric vein thrombosis by ligation, to simulate the pathological process of the disease, and to provide basic preparations for the study of its etiology and treatment.

　　Method: 96 male SD rats were randomly divided into 3 groups, namely group A (sham operation group), group B (strain group), and group C (simple group), each with 32 rats. The rats in group A only opened the abdominal cavity without interrupting the blood supply. The rats were killed in batches at 8, 24, 48 and 72 hours after the operation. Rats in group B and C were ligated to establish superior mesenteric vein thrombosis and strangulation. At 8, 24, 48, 72 hours after modeling, a simple rat model was killed in batches. HE staining is used to observe the morphological changes and injury scores of rat intestinal tissues. ELISA is used to detect the levels of rat serum midgut fatty acid binding protein (IFABP) and α-glutathione S transferase (α-GST).

　　Results: HE staining and pathology scores showed that the degree of intestinal congestion and injury in group B and C were different from those in group A. Group B gradually increased, and group C gradually increased, showing a decline. The degree of bruise injury was positively correlated with the degree of ligation. Serum ELISA results showed that the levels of IFABP and α-GST in the serum of rats in group B and C increased (P≤u003c0.05), and the degree of increase was different compared with group A. as the picture shows. .show. Positive in the range of ligation. relationship.

　　Conclusion: In this study, we successfully established a rat model of thrombosis using ligated superior mesenteric vein. The operation is simple, convenient, and the operation success rate is high, which can be used for related research.