动物造模,高尿酸血症鹌鹑肠道菌群 z-bio 2021-10-14 472

　　Objective: To investigate the structural changes of the intestinal flora of hyperuricemia quail, and to provide reference materials for studying the pathological mechanism of hyperuricemia.

　　Method: Method Difak quails are randomly divided into two groups: the conventional group and the model group. The normal group was fed ordinary feed, and the model group was fed high pudding feed. Two groups of quail cecum were constructed by PCR-DGGE method. Content DNA fingerprint of bacterial flora. Atlas, digitized Atlas, used cluster analysis to compare the overall differences in the structure of the intestinal flora of the two groups of quail, and used logistic regression to identify the difference bands. The sequence of different bands was cloned and identified, and the structural changes of the cecum were observed by HE staining.

　　Result: On the 28th day of modeling, compared with the normal group, the quail blood uric acid of the model group was significantly higher, and the overall structure of the intestinal flora changed. There are 12 different bands on the DGGE fingerprint. The sequence identification belongs to the genus Bacteroides, and the phylum Firmicutes, Proteobacteria and cecal tissues have inflammatory changes.

　　Conclusion: The structure of the intestinal flora of quail with hyperuricemia induced by high-purine diet has changed.