动物造模,免疫细胞分型 z-bio 2021-10-07 487

　　OBJECTIVE: To compare the differences in peripheral blood and immune cell typing indexes between old and young SD rats.

　　Method: We tested the peripheral blood cell count, white blood cell classification, immune organ coefficient, peripheral blood immune cell typing, splenic immune cell typing, and splenic T cell P16 expression in SD rats at 12 months and 8 weeks of age. ..

　　Results: Compared with young rats, the number of white blood cells and lymphocyte ratio in the peripheral blood of old rats decreased, the ratio of red blood cells, hemoglobin, and basophils, the ratio of eosinophils and basophils increased, and the ratios of platelets and basophils increased. The ratio of sex granulocytes increased. There was no difference in the ratio of monocytes, thymus coefficient, and spleen coefficient, and no difference between young and old rats. Monocytes were found in peripheral blood immune cells, helper T cells, regulatory T cells, natural killer cells and aged rats. The proportion of nuclear cells increased and the proportion of B cells decreased. Splenic lymphocyte typing results showed that B cells (CD45RA) in the elderly group were lower than those in the young group (P\u003c0.01), and there was no difference in CD3 between the two groups. The expression of P16 mRNA in spleen T cells of old male rats was significantly higher than that of young male rats (Pu003c0.05).

　　Conclusion: Changes in the typing of peripheral blood cells and immune cells in aged rats. The expression of P16 in spleen T cells can be used as a biomarker of immunosenescence. In this article, we will compare and analyze the test results. It provides basic data for the research of presbycusis and aging animal models.