z-bio 2021-07-05 255

　　Objective: To investigate the genetic and immunological mechanism of guinea pigs' resistance to foot-and-mouth disease virus (FMDV). To clarify the feasibility of FMDV pseudovirus-infected cells for studying the pathogenesis of FMDV.

　　Method: Infect guinea pig primary kidney cells with lentiviral vector containing FMDV-VP1 fragment to construct target cells infected with FMDV pseudovirus. Two guinea pig peripheral blood lymphocyte (PBLC, untreated with antigen) strains were isolated from FMDV sensitive (Zmu-1: DHP strain) and resistant (UK strain), and co-cultured with target cells infected with pseudovirus. Determine the PBLC mortality and use ELISA to detect the cytokine content in the cell culture supernatant. The ratio of CD3 +/CD4 + and CD3 +/CD8 + cells in two guinea pig PBLCs was determined by flow cytometry.

　　Result: For 2 guinea pigs, the cytotoxicity of PBLC in the experimental group was significantly higher than that in the control group (P0.05). The content of PBL CIL-12 and IL-12 in resistant strains of guinea pigs was significantly higher than that of susceptible strains (P\u003c0.05). The lentiviral vector containing the FMDV-VP1 fragment infects primary kidney cells of guinea pigs. FMDV-VP1 protein is expressed on the cell surface; the number of CD4+ cells in the resistant strain guinea pig PBLC is significantly lower than that of the sensitive strain (Pu003c0.05), and the number of CD8+ cells is significantly lower than that of the sensitive strain. The resistant strains are significantly higher. Than sensitive strains (Pu003c0.05).

　　Conclusion: The FMDV-VP1 fragment significantly stimulates the immune response of effector cells and enhances the cytotoxicity of effector cells to target cells. Resistant guinea pig PBLC showed strong natural cytotoxicity to FMDV-infected cells. Effector cells are cytotoxic to target cells. In this process, the cellular immune factors secreted by effector cells participate in related processes. The lentiviral vector containing the FMDV-VP1 fragment was used to infect guinea pig primary kidney cells to construct target cells infected with FMDV pseudovirus for effective simulation. Infect target cells with FMDV. This can improve the safety of your subsequent work. The genetic characteristics of different strains of guinea pigs determine the differences in susceptibility to foot-and-mouth disease virus.