树鼩消化道不同部位微生物 z-bio 2021-07-05 277

　　Purpose: Using tree shrews as a research model to study the composition of the intestinal flora of wild and artificially cultivated tree shrews.

　　Method: Collect 3 healthy wild and artificially propagated tree shrews, take 7 gastrointestinal tract samples, extract the whole bacterial DNA, use high-throughput sequencing technology to generate the V3-V4 hypervariable region of 16SRNA gene, perform sequencing analysis and compare bacteria Community structure and diversity.

　　Result: The analysis of the diversity of alpha index of different intestinal segments showed that there was a significant difference in Shannon index between the wild group and the artificial breeding group of the cecum and stomach (Pu003c0.05). There were significant differences in the microbial composition of various parts of the intestine between the wild group and the artificially propagated group. At the phylum level, the average proportion of Philmictes in the rectum, colon, cecum and stomach of the wild group was significant. It is higher than the artificial propagation group (Pu003c0.05), and the average proportion of spirulina in the rectum, colon, cecum, and ileum in the artificial propagation group is significantly higher than that of the wild group (Pu003c0). 05). The average proportion of Bacteroides rectum is significantly higher In the artificial reproduction group (Pu003c0.05). At the genus level, the wild group was significantly higher than the artificial breeding group. The main bacterial genera are: ileum, jejunum, Weischler, Bacteroides rectum, Lactococcus ileum, and the artificial breeding group. The main bacterial genera are significantly higher than those in the wild group: ileum, colon, cecum, ileum Brevibacterium, and Prevotella in the cecum. _9. Streptococcus lactic acid bacteria in the stomach, cecum and jejunum, and ileum. The prediction of the functional genes of different intestinal flora showed that the differences in 5 anatomical parts and 17 gene categories between the two groups were statistically significant (Pu003c0.05).

　　Conclusion: The composition of the intestinal flora of wild tree shrews and artificially propagated tree shrews is quite different, and the bacterial function of each intestinal segment is also quite different, which is related to its physiological function and diet structure. In the intestinal flora, it is necessary to carefully consider whether the composition of the microorganisms in the stool sample can completely represent the composition of the intestinal flora.