IFITM3基因 z-bio 2021-07-05 346

　　Purpose: To study the function of African green monkey (AGM) interferon-induced transmembrane protein 3 (IFITM) and its antiviral mechanism and the mechanism of action of cloning from various tissues and organs of African green monkey. Analyze the IFITM3 gene and analyze the expression characteristics of the gene.

　　Method: Using RT-PCR method, clone the full-length cDNA of IFITM3 from the heart, liver, lung, kidney, brain and other tissues and organs of African green monkeys. Use DNAMAN software to compare the nucleotide differences of IFITM3 cDNA from different species and analyze the genetic diversity of AGMIFITM3. Real-time fluorescent quantitative PCR was used to detect the expression of IFITM3 gene in various tissues and organs of African green monkeys. An immunohistochemical method for detecting differences in the expression of IFITM3 protein in various tissues and organs of African green monkeys.

　　Result: IFITM3 expression was detected in lymphocytes, skin, heart, brain, liver, kidney, lung and spleen of African savanna monkeys. The obtained IFITM3 cDNA is approximately 440 bp (442-448 bp), which is similar to rhesus monkeys. Monkeys are in terms of genetic evolution. The similarity reached 93? The software predicts that the AGMIFITM3 protein will contain five highly conserved transmembrane domains and specific sites. Real-time PCR results showed that the African green monkey IFITM3 gene was expressed highest in the spleen and lowest in the brain. Immunohistochemistry results showed that the IFITM3 protein content was highest in the sebaceous glands, spleen and lymphocytes. will. The protein content of brain tissue and lungs is relatively low.

　　Conclusion: IFITM3 is widely present in the tissues and organs of African savanna monkeys, and is abundantly expressed in the spleen and lymph of its important immune organs, making it a healthy immune system, which is a powerful for African savanna monkeys immune system. Play an important role in the immunity of African savanna monkeys and their adaptability to important zunotic virus infections.