动物造模,人脐带间充质干细胞 z-bio 2021-07-05 280

　　Objective: To observe the viability of survival, differentiation and embryogenesis of human umbilical cord mesenchymal stem cells (GFP-hUCMSC) injected with green fluorescent protein (GFP) in the liver of SD rats during embryonic stage.

　　The method uses a lentivirus carrying green fluorescent protein to transfect and label human umbilical cord mesenchymal stem cells and inject them into the experimental group of rat embryos. After the birth of the fetus, frozen sections were prepared to observe the migration and distribution of GFP-hUCMSC in the liver tissue, and immunohistochemistry was used to detect the expression of human-related proteins in the mouse liver. The control group was injected with the same amount of normal saline.

　　"Results: The distribution of GFP signal was detected in the liver tissue of the experimental group of fetal mice on the 45th, 75th, and 120th day. The expression of human hepatocyte-related proteins ALB, HNF3β and HNF4α can be detected in the corresponding liver. Although it is tissue, the content of rats at 120 days is very high. The control group was negative.

　　Conclusions The presence of GFP signal and the expression of human hepatocyte-related proteins ALB, HNF3β and HNF4α can be detected in the liver of embryonic mice injected with hUCMSC on the 75th day, and they tend to decrease gradually over time.