Mgst1基因 z-bio 2021-09-23 457

　　OBJECTIVE: To analyze the effect of Mgst1 gene in the process of programmed freezing through the relative expression of Mgst1 gene and the difference of Mgst1 protein before and after program freezing and thawing of mouse hatched blastocysts and dormant embryos. A new theoretical basis for the study of antifreeze mechanism of mouse embryos.

　　Method: Use a laser confocal scanning microscope to collect immunofluorescence images of Mgst1 protein expression and distribution in embryos. Use qRT-PCR technology to identify the relative expression of the Mgst1 gene in the embryo.

　　Results: Immunofluorescence showed that Mgst1 protein was expressed in pre-frozen hatched and resting embryos; qRT-PCR showed that both pre-frozen hatched and resting embryos were expressed. We found that there was no significant difference in the relative expression of Mgst1 gene in embryos (P\→0.05); the relative expression of Mgst1 gene in pelvic leaves hatched after freezing was significantly higher than that before freezing. Regulated (P\u003c0.05); the relative expression of Mgst1 gene in dormant embryos after freezing is significantly up-regulated compared to before freezing (Pu003c0.01) The relative expression of Mgst1 gene in dormant embryos after freezing is similar to that of embryonic cells after freezing The ratio is significantly up-regulated (Pu003c0.05).

　　Conclusion: The Mgst1 gene may play an important positive regulatory role in the antifreeze mechanism of mouse embryos.