动物造模 z-bio 2021-07-06 791

　　Objective: To study the effect of dehydroepiandrosterone (DHEA) on hyperovulation and oocyte quality in C57BL/6J female mice and its mechanism.

　　Method: 303-week-old C57BL/6J female mice were randomly divided into three groups: control group, low-dose treatment group (2mg/kg?D-1), and high-dose treatment group (5mg). /Kg? D-1)). In vitro fertilization was performed by taking DHEA orally for 1 week, and cumulus cells were collected after superovulation. The RNA of cumulus cells was extracted by qRT-PCR, and the mRNA levels of embryo quality-related genes Grem1, Has2, Ptgs1, Ptgs2, Vcan, and apoptosis-related genes Caspase3 and Bcl2l10 were detected.

　　Result: In vitro fertilization (IVF) showed that after excessive ovulation in DHEA-treated female mice, the proportion of atypical eggs in the experimental group decreased. Compared with the control group, the transcription levels of embryo quality-related genes Has2, Ptgs1 and Vcan in the cumulus cells of the experimental group were up-regulated, while the mRNA levels of Germ1 and Ptgs2 were down-regulated. Caspase 3 mRNA levels were down-regulated, and Bcl2l10 mRNA levels were up-regulated.

　　Conclusion: After DHEA treatment, inhibiting the death of cumulus cells can improve the quality of oocytes.