动物造模,荧光RPA检测 z-bio 2021-07-06 497

　　Purpose: In order to improve the surveillance and epidemiological study of port wild and experimental primate monkey T lymphocyte tropism virus type 1 (STLV-1) infection, this study adopts recombinant enzyme polymerase amplification (RPA) and fluorescence method to establish How RPA detects STLV-1, using method software, analyzed the gagpolyprotein gene sequence of STLV-1 isolated from various countries and regions, designed RPA primers and probes, established RPA and fluorescent RPA methods, and detected STLV-1 And verified its sensitivity and stability.

　　Comparison of other specific pathogens in monkeys with STLV-1 confirmed that the established detection method is highly specific. Through the sensitivity test, it is confirmed that the detection limit of the established RPA and fluorescence RPA method is the same as that of PCR. The detection of 30 copies of STLV-1 positive and negative nucleic acid samples confirmed that the established RPA and fluorescent RPA methods have the same stability and reliability as the PCR method.

　　Conclusion The RPA and fluorescent RPA methods established in this study for the detection of STLV-1 have excellent specificity, sensitivity and reliability, and are suitable for STLV-1 surveillance and epidemiological studies.