动物造模 z-bio 2021-07-06 481

　　Purpose: Use ICR mice to conduct in vivo chromosomal aberration experiments, by administering cyclophosphamide (CP) and coxetine (COL) at different time points, and comparing different administration concentrations and different administration/collection times. The effects of chromosomal abnormalities in mice. Bone marrow cells.

　　Method: A total of 36 male ICR mice, approximately 7-8 weeks old, were first randomly divided into a material group 18 hours after administration and a material group 24 hours after administration, and then according to body weight COL 4 mg/kg (COL 4 mg/kg ). 4 hours, ip before sampling) and 10mg/kg (2 hours before sampling, ip) administration group, 0.9? sodium injection as a carrier control, CP 0mg/kg, 10mg/kg, 40mg/kg each group I give Three of them. The weight of the animals before and after the administration was recorded during the experiment. The negative control group and the 10 mg/kg CP group were given twice at 24 hour intervals, and 40 mg/kg CP was given as a single dose. Samples were taken 18 or 24 hours after administration, and COL was injected intraperitoneally 2 or 4 hours before sampling. When collecting materials, all bilateral femurs of experimental animals were collected, bone marrow cell suspension was collected, and Ciemsa chromosome specimens were prepared to analyze the types of chromosomal aberrations and calculate the average structural abnormal cell rate (excluding ctg and csg, AC). ? And structural abnormal cell rate (including eg and esg, ACG?, multiple abnormal cell rate (excluding ctg and esg, MAC? and polyploid cell rate (PC?.

　　Result: The use of CP and COL in this study has no significant effect on animal body weight, and the dosage and frequency of administration are feasible. Compared with the vehicle control group, the AC?ACG?MAC? of all CP treatment groups were significantly increased (Pu003c0.01), and the chromosome damage was more serious at high doses. CP18h-AC?ACG?MAC? in the COL4h group was lower than that in the CP18h-COL2h group (Pu003c0.05), and high-dose COL (10mg/kg) was used to supplement chromosomes. Indicate the damage it may cause. Most types of structural abnormalities in the carrier control group are fissures, and chromosomal monomers and chromosomal breaks are the most important types of abnormalities caused by CP.

　　Conclusion: When conducting chromosomal aberration tests in ICR mice, it is ideal to obtain materials approximately 18 hours after CP administration and approximately 4 hours after COL administration. This research not only accumulates background data through investigating experimental conditions, but also provides references for related researchers.