动物造模 z-bio 2021-07-06 284

　　Objective: To detect the correlation between the SNP locus of SLCIA2 gene and the activity of Labrador and Golden Retriever dogs, and to screen effective genetic and molecular markers for early screening of guide dogs.

　　Method: 99 dogs (80 Labrador and 19 Golden Retrievers) are selected from the guide dog training base in Dalian, China, and the passive test (PT) behavior test method is used to evaluate large-scale events. At the same time, venous blood of 99 dogs was collected, and genomic DNA samples from blood cells were extracted. The three SNP sites of SLCIA2 genes T129C, T1549G, and T47IC were genotyped by PCR/sequencing in 99 dogs. , And analyzed the statistical data of dogs. Correlation between activity and SLCIA2SNP locus.

　　Result: Among the three SNP loci of the SLCIA2 gene, T129C and T1549G have no polymorphisms, only C/C and G/G types respectively. The T47IC locus has three genotypes: T/T, T/C, and C/C. There was no significant correlation between these three genotypes and activity, gender or variety (P\u003c0.05). In addition, there was no significant correlation between breed and activity (P\u003c0.05), but there was a significant correlation between gender and activity (P=0.049).

　　Conclusion: The T129C, TI549G, and T471C sites of SLCIA2 gene have no significant correlation with caterpillar activity, and cannot be used as an index for gene screening of guide dogs. Behavioral testing requires different scoring standards for male and female dog activity, because there is a significant correlation between gender and activity.