动物造模,诺如病毒 z-bio 2021-09-26 343

　　Objective: To study the histopathological changes and the changes of virus content in commonly used experimental mice after artificial infection with norovirus.

　　Method: Five strains of mice, KM, BALB/c, NIH, C57BL/6, and BALB/c-nu, were used to divide each strain into a control group and an infection group. Inoculate the infected group with oro. The virus-fed control group was injected with the same amount of normal saline at 7, 14, 21, 28, and 55 days after infection, and the liver, spleen, lung, cecum, colon, and small intestine were taken. ..

　　Results The total incidence of liver, spleen, and lung lesions was 8-10/125), 5.6-7/125) and 4-5/125). No lesions were seen in the cecum. The lesions of KM and BALB/c mice mainly occurred in the liver, spleen and lung, the lesions of C57BL/6 mice mainly occurred in the liver and lung, and the lesions of IH and BALB/c-nu mice mainly occurred. Located in the liver and spleen. Among the observed experimental mice, KM mice are more prone to lung lesions, KM and NIH mice develop earlier lesions, and C57BL/6 mice develop later. The positive rate of MNV nucleic acid in the cecum and colon of all experimental mice was 100125/125), and the positive rates of small intestine, liver, spleen, lung and blood were 71.2±89/125 and 17.622/125, 39.2, 39.2, ?? 9/125), 3.2? /125), 1.6? /125); Comparison of virus content: brain intestine → colon → small intestine → spleen → lung → liver; BALB/cu mouse has a significantly higher virus content than other mouse strains (Pu003c0.01), which is not statistically different from other strains of mice Virus content in various tissues; C57BL/6 mice (Pu003c0.05) reached peak values at different times in intestinal and colon virus content on day 14 of MNV compared with other strains. Virus content, physical and tissue and organ disease examination results, and nucleic acid test results of different infected mice.

　　Conclusion Norovirus infection in mice can cause tissue damage. We recommend choosing MNV-negative mice for pathological animal studies.