动物造模,巨细胞病毒 z-bio 2021-09-26 763

　　Objective: To establish a purification method for mouse cytomegalovirus from uninfected MCMV mice.

　　Method: Use embryo transfer technology, use different hormones, inject hormones during different estrus cycles, recipient mouse strains, different transplant methods, different embryo transfer stages, and during transplantation, and optimize purification conditions according to the number of embryos. Purify donor mice using optimized embryo transfer purification methods.

　　Result: The number of embryos obtained through ovulation at a dose of 10 IU of a hormone produced by SIGMA is about 17 per mouse, and the number of embryos obtained through ovulation during the period of interest in the mouse is the largest. Only about 23 embryos/only embryos can be used for excessive ovulation; the offspring of C57 male mice mated with ICR female mice are selected as recipient mice, and the litter rate reaches 44.5. The effect of ovulation transplantation is better than that of uterine transplantation. The litter rate is 40.64? The conception rate of 2-cell embryos is 80? It is significantly better than single-cell and 8-cell embryos. After 24 embryos are transferred to recipient mice, the birth rate reaches 43.75? Optimized mouse cytomegalovirus embryo transfer The purification method is used to purify mice without MCMV infection.

　　Conclusion: A purification method for mouse cytomegalovirus has been established. This ensures that the mouse population can be obtained without being infected with MCMV.