动物造模 z-bio 2021-07-06 394

　　Objective: To clarify the expression position and level of TRIM26 in human and mouse tissues and organs.

　　Methods: Immunohistochemistry PV-9000 two-step method (non-biotin) was used to study the expression site, and the BioGPS database was used to analyze the expression level of TRIM26 in human tissues, and RT-qPCR was performed to detect the expression level of TRIM26 in the tissues in the main research. . Mouse organs.

　　Results: IHC results showed that TRIM26 protein signal localizes in the cytoplasm and nucleus, and its expression is more common in epithelial cells. TRIM26 protein exists in human and mouse esophageal squamous epithelial cells, gastric epithelial cells, small intestinal epithelial cells, colon epithelial cells, pancreatic islet cells, brain glial cells, renal tubular epithelial cells and glomerular cells, and endometrial glandular epithelial cells in. can be seen. Consistent with... In addition, TRIM26 has the highest relative expression in human airway tissue and mouse spleen tissue.

　　Conclusion: The expression position of TRIM26 in human and mouse tissues is the same, and the expression level is slightly different. The clear expression profile of TRIM26 in human and mouse major tissues and organs provides an excellent basis for using TRIM26 conditional knockout mice to analyze the function of TRIM26 in various tissues and organs.