动物造模 z-bio 2021-09-26 331

　　Objective: To obtain the full-length coding sequence of adhesion molecule A, analyze its molecular characteristics, and the mechanism of JAM-A as a leovirus receptor to invade and adhere to primary lung cells.

　　Method: Extract total RNA from healthy ferret tissue, and use reverse transcription PCR and cDNA endorapid cloning technology to obtain JAM-4 gene. UniproUGENE analyzed the encoded amino acids and constructed a phylogenetic tree using MEGA 6.0 software. Using the maximum likelihood method, real-time fluorescent quantitative PCR was used to detect the expression and distribution of JAM-A in 25 tissues and blood of trees. Primary lung epithelial cells were found in primary lung receptors. Specific antibodies blocked tree epithelial cells and then used To observe the effect of viral antigens on Leo. The impact of orphan virus infection. The result of the full-length cDNA sequence of JAM-A gene is 2962 bp. Phylogenetic tree evolution analysis shows that the genetic relationship between JAM-4 gene and human is closer than that of rodents. The JAM-A molecule is the peripheral organization of the tree. The expression level is very high in the respiratory and gastrointestinal tract. The specific antibody acts on the cell and significantly reduces the integrated immunofluorescence absorbance of the virus antigen (Pu003c0.0001).

　　Conclusion: The first cloning and analysis of the JAM-A sequence confirmed that the JAM-A molecule is the main receptor for the reovirus to invade trees, and the tree can be used as a new animal model of the Reoviridae family.