动物实验 z-bio 2021-07-07 712

　　Objective: To evaluate the feasibility and safety of two different left atrial appendage occluders (single-disc cylindrical type and double-disc split type) for blocking the left atrial appendage of the target animal.

　　Method: 14 healthy experimental dogs used a single-disc cylindrical or double-disc split left atrial appendage occluder to occlude the left atrial appendage through the femoral vein, and perform intraoperative angiography of the left atrial appendage to evaluate the effect. The accidental death experiment immediately carried out macroscopic specimen inspection to analyze the cause of the dog's death. The surviving experimental dogs were sacrificed 45, 80, 110 and 15 months after the operation. The occluded surface tissue was stained with HE, DAPI and CD31 immunofluorescence. Assess the degree of endothelialization.

　　Results: Six experimental dogs used a single-disc cylindrical occluder to treat left atrial appendage obstruction. Five experimental dogs died 30 minutes to 8 hours after surgery. One experimental dog survived for 15 months and I was put to death. Blood clots on the surface of the experimental dog occluder survived. About one-fifth of the surface of the experimental dog occluder was not covered with new tissue; 7 experimental dogs were completely occluded, and the new tissue completely covered the surface of the occluder 110 days after the operation, HE and immunofluorescence Staining shows intact intima formation and new blood vessels can be seen.

　　Conclusion: After the single-disc cylindrical occluder is transplanted to the left atrial appendage, the incidence of acute thrombosis and immediate mortality are both high. Use a double-disc split left atrial appendage occluder to block the left atrial appendage device. Related complications After the left atrial appendage low-position closure operation, the 4-mesh intervertebral disc is completely intact.