[Animal Modeling]-Isolation, Culture and Identification of Bacteria from Tree Shrew Feces

  Objective: To understand the diversity of the fecal flora of artificially-bred tree shrews, and to provide a basis for the normal breeding of tree shrews and the standardization of microbial quality control.

  Method: Randomly extract 10 fecal samples from Tupai, separate and cultivate bacteria with aerobic and anaerobic media, extract bacterial genomic DNA, and perform PCR amplification and sequencing of 16SRNA gene.

  Results: In this experiment, 25 strains and 12 strains of bacteria were isolated and identified from Shigella stool samples in aerobic culture. Proteus, Enterococcus, and Escherichia were contained in anaerobic culture, and 25 were isolated and identified. Strains of bacteria and 10 strains of bacteria. Shigella, Staphylococcus, Anaerobic bacteria, Laurel bacteria, Laurel bacteria, Microbacteria, Streptococcus, Interleukin

  Conclusion: Tree shrews are rich in aerobic and anaerobic bacteria. The main intestinal parasites of the tree shrew group may be Proteus, Ferguson Escherichia coli and Enterococcus faecium.