动物造模 z-bio 2021-07-08 648

　　Objective: To carry out forward genetic screening of mutants with developmental disorders of zebrafish liver, intestine and gallbladder.

　　Method: ENU mutant wild-type zebrafish is used for classic F2 production screening, whole embryo in situ hybridization using lfabp as a probe, and early zebrafish embryo liver, intestine and gallbladder detection. Perform BES-H2O2-Ac fluorescent dye. Phenotype.

　　Result: 23 zebrafish gastrointestinal development defect mutants of 14 F2 families were screened out of 128 mutant genomes, and they were divided into 6 categories according to their phenotypes.

　　Conclusion: The developmental regulation mechanisms of zebrafish liver, intestine and gall bladder are similar and different.