动物造模 z-bio 2021-07-08 944

　　Objective: To investigate the role of ripply1 gene in the early development of dorsoventral axis in zebrafish.

　　Methods: Using zebrafish whole cyst in situ hybridization technology to reveal the expression pattern of ribply1 gene in the early embryonic development of zebrafish, and using microinjection technology to produce ribply1 mRNA at the embryonic cell stage 1, and inject high-expressing Ripply1 protein. Observation stage Changes in embryonic dorsal and abdominal marker genes and embryo morphological changes. Using Tol2 transgenic technology, we constructed a GFP transgenic fish driven by ribply1 promoter.

　　Result: The result of in situ hybridization indicated that ribply1 gene was specifically expressed in the early embryonic shield stage of zebrafish stomach, that is, the embryonic shield on the given back. After the high expression of ipply1, the expression range of marker genes expanded after the blastocyst stage, and the expression of abdominal marker genes was weakened. Twenty-four hours after fertilization, the embryo exhibits a severe dorsal phenotype: the head increases, the yolk extension of the abdomen decreases, the tail trunk and tail increase, and the area decreases, and some of the second individuals even form an axis. The resulting transgenic fish revealed the maternal expression of ipply1, and GFP driven by 1,200 bases upstream of the transcription start site can mimic the expression pattern of endogenous genes.

　　Conclusion: Ripply1 may be involved in the early development of the dorsoventral axis of the zebrafish embryo.