动物造模,肾动脉栓塞 z-bio 2022-02-05 1147

　　OBJECTIVE: To establish a canine renal artery embolization model with microsphere embolization, and evaluate the effect and local histology of unilateral renal artery embolization in dogs.

　　Method: The experimental animals were 8 beagle dogs, immediately after embolization, they were divided into 4 weeks, 12 weeks, and 26 weeks groups, and 2 dogs in each group were selected from the microsphere embolization group. There is also a normal control group. First, all left renal artery embolizations were embolized with microspheres. Immediately after embolization, renal angiography and DSA confirmed the changes after renal embolism at 4, 12, and 26 weeks. Then, the experimental animals were sacrificed and the kidneys were observed for histopathology.

　　Result: Gross pathology: Compare the size of the embolized kidney with the size of the contralateral kidney. The kidneys gradually shrink at 4, 12, and 26 weeks after embolization. The longer the embolization time, the smaller the volume. .. Obvious; the color of the embolized kidney becomes brighter and uneven; the surface of the embolized kidney is gradually smooth and uneven, and the kidney capsule is difficult to separate. Observation under light microscope: 4 weeks, 12 weeks, and 26 weeks after embolization; microsphere filling can still be seen in the small branches of the arcuate artery, arterial wall vitrification, and arterial fibrosis; embolization time is long, and as time goes by, there are many degenerative tissues Changes, including early renal atrophy, degeneration, and old renal infarction. There are many progressive changes in kidney tissue in the later stage, including pathological changes such as renal tubular regeneration, granulation and remodeling. It will change. However, the embolized kidney has almost normal glomeruli and tubular structures.

　　Conclusion: The microsphere embolization agent has a certain embolization effect on the unilateral renal artery in dogs.