动物造模 z-bio 2021-11-09 366

　　OBJECTIVE: To observe the effect of individual electric shock stress time on elevated cross maze and anxiety-like behaviors in open experiments in rats.

　　Method: Adult male SD rats were randomly divided into a control group and a single ECG group according to their body weight, with 12 rats in each group. The sole electric shock group received the inevitable sole electric shock (0.8 mA, electric shock 10 seconds, rest 20 seconds, total 5 minutes, 1 time/day) combined with orphan breeding to establish a rat anxiety model. The elevated plus maze and open experiments were measured 7 days and 14 days after a single electric shock to evaluate the rats' anxiety behavior.

　　Result: 7d and 14d after the plantar electric shock, anxiety-like behavior increased in the plantar electric shock group, the difference was significant (Pu003c0.01), and the total number of arm pathways was significant in the control group. less than. Physical activity has decreased. In the open experiment, the number of times of invading the central part and the staying time in the central part of the single electrosensitive group were shorter than those of the control group (Pu003c0.01), and the number of exercises and the number of posts were less than those of the control group (Pu003c0.01). , And caring less in the control group. Significantly lower than (P\u003c0.01). Sports exploration activities are decreasing. Comparing the behavioral results of the 7-day and 14-day plantar shock groups, we can see that as the modeling days increase, the time ratio of the rat's closed arm frequency is displayed. The downward trend (Pu003c0.05) and the degree of anxiety decreased. In the start-up experiment, the total exercise distance, standing times and caring times were all 14 days shorter than 7 days, and the difference was significant (Pu003c0.05), indicating that the exercise-seeking behavior is further reduced by rats. Self-attention decreases and depression-like behaviors increase.

　　Conclusion: Different time intervals of plantar electric shock stress can cause anxiety-like behaviors in rats, which affects rats' exercise exploration activities. As the number of modeling days increases, the behavior of rats will change from things like anxiety to things like depression. The delay selection of a single electrosensitive stress anxiety model is 7 days better than 14 days.