动物造模,糖尿病大鼠心肌纤维化 z-bio 2021-07-12 225

　　Objective: To investigate the effect of hydrogen sulfide (H2S), a gas signal molecule, on myocardial fibrosis and the protein expression of MAPK1/3 and MMP-8 in diabetic rats.

　　Methods: 40 SD rats were randomly divided into 4 groups: normal control group, diabetic rat group (STZ group), hydrogen sulfide intervention diabetic rat group (STZ+H2S group) and hydrogen sulfide intervention normal rat group (H2S group) ). Rats were intraperitoneally injected with streptozotocin (STZ) (40 mg/kg) to establish a diabetes model; the control group was intraperitoneally injected with normal saline every day; the STZ+H2S and H2S groups were treated with hydrogen sulfide and sodium hydrogen sulfide (H2S donor). Daily intraperitoneal injection of 100 μmol/kg solution, 8 weeks later, the rats were sacrificed, HE staining to observe the pathological changes of myocardial fibers, type I collagen, MAPK1/3, Western blot to detect the expression of MMP-8 protein.

　　Result: The collagen content in the myocardial tissue of the diabetes group was significantly higher than that in the control group, and the expression levels of myocardial interstitial fibrosis, type I collagen, MAPK1/3 and MMP-8 protein were significantly increased. 05).

　　Conclusion: H2S may improve myocardial fibrosis in diabetic rats, and its mechanism may be related to the inhibition of MAPK1/3/MMP-8 signaling pathway.