动物造模,哮喘小鼠气道高反应性 z-bio 2021-07-12 357

　　Objective: To investigate the effect of chloroquine on airway hypersensitivity in asthmatic mice and its possible mechanism.

　　Method: Balb/c mice were randomly divided into control group, asthma group, chloroquine treatment group, dexamethasone treatment group, and chloroquine + dexamethasone treatment group. Except for the control group, all mice in the other groups were given OVA. Attack, establish a mouse model of asthma, intraperitoneal injection before each attack, the control group is treated with the same dose of PBS at the same time, the mouse lung function device detects the airway hyperresponsiveness of the mouse within 24 hours after the last attack. The total number of cells in bronchoalveolar lavage fluid (BALF) and their classification number were detected, and pathological sections of lung tissues were prepared. After HE staining, the pathological changes were observed by optical microscope. Use ELISA to detect the level of cytokine IL-. 6 and PGF2α in bronchoalveolar lavage fluid.

　　Results: Chlorokin can significantly reduce airway hyperresponsiveness in asthmatic mice (4.5±0.4 vs 6.1±0.9 when inhaled 25 mg/mlAch, 4.6±0.5 when inhaled 50 mg/mlAch, Pu003c0.05, vs8.2± 1) Pu003c0.001); significantly reduce the number of inflammatory cells in bronchiolar lavage fluid of asthmatic mice (Pu003c0.01); significantly reduce the pathological score of asthmatic mice (Pu003c0.05); inflammation of bronchioles and asthma The peribronchiolar (Pu003c0.05) of mice, combined with dexamethasone, can reduce the level of PGF2α factor in BALF of asthmatic mice, perivascular inflammation (Pu003c0.01), and lung histopathology score (Pu003c0.01) 001 ) Can be significantly reduced, and IL- can be significantly reduced. In the BALF of asthmatic mice. 6 factor level (Pu003c0.05).

　　Conclusion: Chloroquine can inhibit airway hyperresponsiveness in asthmatic mice. The combination of dexamethasone and chloroquine is more effective than a single drug and may be effective for asthma involving neutrophils.