动物造模 z-bio 2021-07-12 433

　　Objective: To investigate the role of up-regulation of KA1 subreceptor expression in the death of hippocampal neurons caused by endoplasmic reticulum stress.

　　Methods: 70 adult male kelp mice were randomly divided into kainic acid group (KA group), tunicamycin group (TM) 500 μg/ml group, TM 1000 μg/ml group, TM 2000 μg/ml group, and Inject into the hippocampus. Different concentrations of KA or TM in different time periods (1, 2, 3, 4, 5, 8, 12 hours). Before perfusion, the brains were harvested for Bederson marker scoring, FJB staining, and immunohistochemical analysis of whole brain sections.

　　Results: At 3, 4, 5, and 8 hours in the KA group and 4 and 5 hours in the TM 2000 μg/ml group, the Bederson sign score indicated that the central nervous system function was significantly impaired. FJB staining showed that hippocampal cell death KA1P-eIF2α and P-eIF2α Significantly up-regulated in hippocampal neurons (Pu003c0.05).

　　Conclusion: After injection of KA or TM into the hippocampus, the results indicate that KA1 is expressed through endoplasmic reticulum stress. In the process of neuronal apoptosis, the surface membrane receptor KA1 may first receive the apoptosis signal and transmit it to the cell. Endoplasmic reticulum dysfunction induced. It causes endoplasmic reticulum stress and further promotes neuronal apoptosis.