动物造模,小鼠黑色素瘤细胞 z-bio 2021-07-12 740

　　Objective: To study the effect of high-intensity focused ultrasound (HIFU) treatment on melanoma cell metastasis in a mouse model of melanoma.

　　Method: Mouse melanoma cells B16-F10 were injected subcutaneously to construct a mouse melanoma subcutaneous transplantation tumor model. When the longest diameter of the tumor grows to 7-10mm, HIFU treatment is performed and an experimental control group (with cancer) is set. Perform mouse radiation HIFU, but turn off the power switch on the treatment head. That is, the fake group. After treatment, the large and small diameters of the tumor were measured every 3 days, and the tumor curve was drawn for 3 weeks. The relative expression of the three markers of melanoma cells in the blood, survival and metastasis are calculated and quantified by real-time fluorescence, and measured by PCR to monitor the number of circulating melanoma cells in the blood. These are all related to melanoma. Antigen A3 (MAGE-A3) and T cell 1 (MART1) recognize melanoma antigens) and homologously convert them into the pair-box gene transcription factor PAX3); 14 days after HIFU treatment, B16-F10 cells were injected and the lung metastasis rate was observed-by Tail vein transplantation.

　　Results: (1) Median survival time and 95 confidence interval (CI): The fake photo group was 19.00d and 17.14-20.86d, and the HIFU group was 26.00d and 24.76-27.25d. Significant survival rate

　　arrived (Pu003c0.01); two groups of mice, starting 20 days after treatment, 20 days after HIFU treatment, 23 days and 26 days after treatment, there was a significant difference in tumor volume including irradiation. The tumor volume of mice in the HIFU group was significantly smaller than that in the sham group (Pu003c0.05); (2) The real-time fluorescent quantitative PCR results showed that the three indicators of MAGE-A3, MART1 and PAX3 were HIFU, and the group was significantly reduced 7 days after treatment. The expression of MAGE-A3 in the fake photo group (Pu003c0.05) 14 days after treatment was significantly lower than that of the fake photo group (Pu003c0.05). 0.05); (3) 14 days after HIFU treatment, tail vein retransplantation of the same tumor, the number of lung metastases in the HIFU group was significantly lower than that in the sham group (Pu003c0.05), and the tumor metastasis inhibition rate was 42.4?

　　Conclusion: HIFU treatment can inhibit the development of melanoma cell metastasis in vivo. The mechanism is that tumor cells fall off from the primary tumor and the ability of tumor cells to colonize the lungs may include a reduction in the ability of tumor cells to colonize the lungs.